[BioC] marrayPlot
Niklas Nordquist
niklas.nordquist@genpat.uu.se
Fri, 24 May 2002 02:18:05 +0200
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Hi,
I've just started using "R" with the bioconductor-packages. I'm now
trying to analyse our cDNA-array data.
Using the function "read.Spot" I've managed to import my data from a tab
separated file. After this, I've used the normalization function
"maNormMain(mbatch=TOR493, f.loc=list(maNormLoess(x="maA", y="maM",
z=NULL, w=NULL, subset=TRUE, span=0.4)), f.scale=NULL,
a.loc=maCompNormEq(), a.scale=maCompNormEq(), echo=FALSE)".
So far everything seems to be fine, but when I try to visualize my
results with "maDiagnPlots1(mraw=TOR493, title=NULL, save=FALSE)",
I get an error message "Error in rep(NA, maNspots(L)) : invalid number
of copies in "rep"". I don't understand this error message. What could
be wrong?
I'd appreciate if someone who has more experience than me could give me
any suggestions.
Regards,
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<p class=3DMsoNormal><font size=3D2 face=3DArial><span lang=3DSV =
style=3D'font-size:10.0pt;
font-family:Arial;mso-ansi-language:SV'>Hi,<o:p></o:p></span></font></p>
<p class=3DMsoNormal><font size=3D2 face=3DArial><span lang=3DSV =
style=3D'font-size:10.0pt;
font-family:Arial;mso-ansi-language:SV'><o:p> </o:p></span></font></=
p>
<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt;
font-family:Arial'>I’ve just started <span class=3DGramE>using =
”</span>R”
with the <span class=3DSpellE>bioconductor</span>-packages. I’m =
now trying
to <span class=3DSpellE>analyse</span> our <span =
class=3DSpellE>cDNA</span>-array
data.<o:p></o:p></span></font></p>
<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt;
font-family:Arial'>Using the function “<span =
class=3DSpellE>read.Spot</span>”
I’ve managed to import my data from a tab separated file. After =
this,
I’ve used the normalization function “<span =
class=3DSpellE><span
class=3DGramE>maNormMain</span></span><span class=3DGramE>(</span><span
class=3DSpellE>mbatch</span>=3DTOR493, <span =
class=3DSpellE>f.loc</span>=3Dlist(<span
class=3DSpellE>maNormLoess</span>(x=3D"<span =
class=3DSpellE>maA</span>",
y=3D"maM", z=3DNULL, w=3DNULL, subset=3DTRUE, span=3D0.4)), =
<span class=3DSpellE>f.scale</span>=3DNULL,
<span class=3DSpellE>a.loc</span>=3D<span =
class=3DSpellE>maCompNormEq</span>(), <span
class=3DSpellE>a.scale</span>=3D<span =
class=3DSpellE>maCompNormEq</span>(),
echo=3DFALSE)”.<o:p></o:p></span></font></p>
<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt;
font-family:Arial'>So far everything seems to be fine, but when I try to
visualize my results with “<span =
class=3DGramE>maDiagnPlots1(</span><span
class=3DSpellE>mraw</span>=3DTOR493, title=3DNULL, =
save=3DFALSE)”,<o:p></o:p></span></font></p>
<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt;
font-family:Arial'>I get an error message “Error in <span =
class=3DGramE>rep(</span>NA,
<span class=3DSpellE>maNspots</span>(L)) : invalid number of copies in
"rep"”. I don’t understand this error message. =
What could
be wrong?<o:p></o:p></span></font></p>
<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt;
font-family:Arial'>I’d appreciate if someone who has more =
experience than
me could give me any suggestions.<o:p></o:p></span></font></p>
<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt;
font-family:Arial'>Regards,<o:p></o:p></span></font></p>
<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=3DMsoNormal><font size=3D2 face=3DArial><span =
style=3D'font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
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