[BioC] Quality of Affy Chips with the affy package
Thu, 20 Feb 2003 18:26:10 +0100
> 'image' lets you do a visual inspection of the chip
> (scratches, air bubble(s),
> washing step problem, ..., should be visible).
> 'hist' can let you see saturation (if you have the
> distribution of probe
> intensities showing a strange 'bump' on the higher end of the
> intensity range,
> you may suspect that the captor (a CCD camera I believe) get too much
> an cut off the signal. You can confirm with a scatter plot (or matrix
> of scatter plots (see pairs.AffyBatch))).
> 'boxplot' and 'hist' can let you vizualize the effect of normalization
> for example (quality control on the processing step).
if I find such areas with scratches, bubbles or saturation on a chip,
how can I mask them?
Also, if probe level intensities go into saturation, how does that
affect signal values? I ask, because there will generally be probes in a
probe set that won't have that problem. Sure, this will depend on the
signal value estimation method. How do RMA and MBEI react when some
probes ru in saturation?