[BioC] Re:MAGEML

sdurinck Steffen.Durinck at esat.kuleuven.ac.be
Thu Nov 6 12:29:54 MET 2003


Hi Cary,


There should be no problem to import this dataset.  In ArrayExpress
you'll find most of the time two files that are related to a certain
experiment.  One contains the experiment data and begins with an 'E' .
In your case this is E-CAGE-*.  This is also the file you find when
searching for an experiment via ArrayExpress.  The second xml file
contains all information on the ArrayDesign and begins with an 'A' 
you'll find this file by clicking on the link 'Array design used' which
you'll see when you searched for an experiment.
For you this is A-MEXP-10.

So now you'll have two MAGEML files from which the combined information
results in a marrayRaw object.

First get the array design info and  make a layout and gnames object
with the command widget.readMageDesign.  Here you'll have to point to
both files, see manual. You can use the show packages button to see if
the package that is required is present in the file you pointed to with
browse.

Secondly make the raw object by using widget.readMageRaw.
Here you'll only have to use the xml file from  E-CAGE ...

Let us know if you still run into problems.

Cheers,
Steffen & Joke


-------------------------------------------------------------------

Hi all,
  I am attempting to use public MAGEML data without success.  My current
datasets are E-CAGE-{2,3,4} from ArrayExpress.  According to the tutorial
a MAGEML file requires packages
BioAssayData
BioAssay
BioMaterial
BioSequence
ArrayDesign
DesignElement

I suppose this is the problem because inspection of package content shows
this:
> magePackageDetector(ecage3)
 
 File  aVIB-Col_vs_aUNIL-Col.xml  contains the following packages:
 
         BioMaterial_package
         BioAssay_package
         QuantitationType_package
         BioAssayData_package




There is only 1 xml file included with the data.  How can I get the
correct packages?   Any help will be appreciated.  Thanks
  --cam

-- 
//=\ Cary Miller                                    //=\ \=// Center for
Computational Pharmacology          \=//
//=\ University of Colorado Health Sciences Center  //=\ \=//
http://compbio.uchsc.edu/Hunter_lab/Miller     \=//
//=\ (303) 315-1584                                 //=\



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