[BioC] RNA digestion plots
Peter.Robinson at t-online.de
Wed Nov 26 00:38:40 MET 2003
I am looking for help in the interpretation of RNA digestion plots. If I have
understood the recent discussions, then one expects to see a trend from 3' to
5' in average probe intensity.
I am comparing two sets of experiments done in two different labs. Both were
done with mgu74av2 chips.
One set is basically flat with a slight drop at the very 5' end (probe number
0) and a large drop at the very 3' end (probe number 15), while the other
shows a slow increase in intensity from the 5' to the 3' end except for a
drop at the very 3' end (probe 15).
Does this indicate that the second set may exhibit problems with RNA
Also, I cannot seem to find the article:
"L. Cope. Detecting rna degradation using probe level data from
oligonucleotide expression arrays. Bioinformatics, 2003"
in pubmed. (This article is listed on the bioconductor site.) Could anybody
point me to a url to get this article (or perhaps a preprint?)
Institute of Medical Genetics
Charite University Hospital
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