[BioC] read subset of affy data

Rafael A. Irizarry ririzarr at jhsph.edu
Sat Oct 18 22:46:26 MEST 2003


you can use read.probematrix directly (its what justRMA calls). 
it reads either only pm, only mm, or both without creating an
AffyBatch.


On Sat, 18 Oct 2003, James MacDonald wrote:

> Look at the code for justRMA in the release version of affy. It is much
> less memory intensive to iteratively put a cel file in an affybatch,
> extract the PM data to a matrix and then overwrite the affybatch with
> the next cel file. You could easily modify the code to only extract the
> PM data you are interested in into the matrix.
> 
> Alternatively you could simply use justRMA to get expression values and
> go from there.
> 
> HTH,
> 
> Jim
> 
> 
> 
> James W. MacDonald
> Affymetrix and cDNA Microarray Core
> University of Michigan Cancer Center
> 1500 E. Medical Center Drive
> 7410 CCGC
> Ann Arbor MI 48109
> 734-647-5623
> >>> Xuejun Peng <xpeng at bio.ri.ccf.org> 10/17/03 19:22 PM >>>
> I have 100 affy arrays and I got an "out-of-memory" error message when I
> 
> try to batch-read all of them simultaneously. On the other hand, it is 
> really inefficient to read them one by one.
> Since I have a specific list of genes that I want to read, can I use 
> ReadAffy or some other functions to read the subset of genes directly 
> from .cel files? For a simple example, I only want to read  five genes 
> and I know their probe set ID's.
> 
> Can anyone help? Thanks.
> 
> Xuejun
> 
> 
> -- 
> Xuejun Peng, Ph.D.
> Assistant Staff
> Biostatistics and epidemiology / Wb4
> Cleveland Clinic Foundation
> 9500 Euclid Avenue
> Cleveland, OH 44195
> Phone: 216-444-9958
> Fax: 216-444-8023
> E-mail: xpeng at bio.ri.ccf.org, pengx at ccf.org
> 
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