[BioC] OT: cell lines and tissues
YUK FAI LEUNG
yfleung at mcb.harvard.edu
Fri Jul 9 17:15:02 CEST 2004
Why don't you use both of them as controls? A group of RNAs from normal
amplified RNA from tissue, and a group of RNAs from cell lines. Then you
can make more comparisons!
This is definitely Off-Topic, but I'd like to have an opinion from the
many biologist (but not only!) that populate the list, about the
A group of biologists is willing to study gene expression in ovarian
cancer tissues relative to normal ones. As the normal ovarian epithelium
is single layer, it's quite hard to get enough RNA. So
they are actually going to compare normal ovarian cell lines grown in
vitro versus patological tissues. I feel a bit confused about this.
Wouldn't be better to amplify the RNA from normal
tissues? Any other options?
Any insight will be very appreciated.
Yuk Fai Leung
Department of Molecular and Cellular Biology
BL 2079, 16 Divinity Avenue
Cambridge, MA 02138
email: yfleung at mcb.harvard.edu; yfleung at genomicshome.com
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