[BioC] Kooperberg introduces Nas??
michael watson (IAH-C)
michael.watson at bbsrc.ac.uk
Fri Jun 4 15:39:33 CEST 2004
I have read in some data using the limma examples and everything is
fine, I have used the kooperberg algorithm and everything works, APART
from one single spot where I have an NA for some reason:
 255 919 371 315
 152 929 116 NA
Now, the spot in question has a Cy3 foreground mean of 2256 and a Cy3
background median of 4782.
OK, so it is a bad spot. But GenePix hasn't flagged it, perhaps because
background is only high in the Cy3 channel - whatever.
Anyway, I didn't realise that Kooperberg could introduce NAs into the
data set - I thought it was this type of problem that kooperberg was
meant to address? (ie negative spots).
Thanks in advance for your help
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