[BioC] ULYSIS Alexa Fluor labelling kit / dye bias / limma

[Matthew Hannah]

Hi,

Quick question this time. Basically these dyes have higher intensity,
less quenching and correlate more between dye pairs. The ULYSIS kit uses
chemical labelling which eliminates sequence effects.

http://www.probes.com/servlets/directory?id1=6&id2=48&id3=319
http://www.probes.com/media/publications/394.pdf

As far as I know Limma accounts for dye bias during normalisation. If
there is less/no bias will there be artifacts introduced? Or other
points I need to be aware of?

Thanks,
Matt