[BioC] Re: Advice on print-tip normalization
Gene Cutler
gcutler at amgen.com
Thu Sep 23 18:27:22 CEST 2004
On Sep 22, 2004, at 6:11 AM, Reimers, Mark (NIH/NCI) wrote:
> Hello Gene,
> I don't have any advice but some related observations based on looking
> at regional biases on spotted microarrays. In the slide data that have
> come in, there seems often to be a bias toward red on the top and
> bottom edges of the print-tip groups, and a bias toward green in the
> middle of the print-tip blocks. No explanation occurs to me, but this
> effect is apparent on most of the arrays. One of our collaborators
> claims the effect disappears with a more effective washing treatment,
> but hasn't sent slide images.
>
What I'm seeing are largely overlapping periodicities in both channels.
The intensities start low at the beginning of a block, peak in the
middle, and go down again at the end. This repeats in each of the 48
blocks across the slide, getting more exaggerated across the array.
This is accompanied by a greater scatter in the intensity ratios, with
much more noise in the center of the grids, where both channel
intensities are highest.
Gene Cutler
Research Investigator
Bioinformatics
Amgen SF
1120 Veterans Blvd.
South San Francisco, CA 94080
(650) 244-2489
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