[BioC] Spot quantitation

Jason Skelton jps at sanger.ac.uk
Wed Apr 27 14:12:47 CEST 2005


I was wondering if anyone has any opinions about the SPOT spotfinding 
software from csiro
(I.e the package that runs in R) and it's limitations, benefits, opinion 
about batch performance accuracy etc.
Has anyone done any direct comparisons of SPOT with Genepix or other 
spotfinding software avalible ?
Or is anyone routinely using SPOT and highly recommend it...
I'm currently comparing it to genepix data from a relatively old version 
of genepix with kooperberg background subtraction
and using limma for all subsequent analysis.

many thanks


Jason Skelton
Pathogen Microarrays
Wellcome Trust Sanger Institute
CB10 1SA

Tel +44(0)1223 834244 Ext 7123
Fax +44(0)1223 494919

More information about the Bioconductor mailing list