[BioC] RNA deg plot
jjin at unc.edu
Thu Apr 28 19:32:17 CEST 2005
I looked at RNA degradation for a hg-U133plus2 chip, which a lab ran for a
pilot experiment(please see the attachment). The RNA digestion plot
appeared fine except the 5' end probe and a couple of 3' end probes. The
mean intensity on both end positions dropped dramatically relative to
I have observed several other RNA digestion plots of hg-U133plus2 chips run
by different labs. These plots are almost linear with slope ranging from 3
to 6. Most often it was 5' end probes that underwent significant RNA
degradation. Does anyone have reasonable interpretation for the "drop down"
plot? Any comments are highly appreciated.
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