[BioC] GCRMA error

Zhijin Wu zwu at jhsph.edu
Fri Feb 11 14:22:48 CET 2005


can you try rma and see if you get error too?

rma.cold.control <- rma(raw.cold.control)
 
from your output it seems upto normalizing it is working OK. After that
the function is the same as rma and actually calls rma to calculate
expression. 

best,
Jean
On Fri, 11 Feb 2005, Dion Whitehead wrote:

> Hi,
> 
> I have an unidentied error in the gcrma package.  I'm stuck on the cause:
> 
>  > gcrma.cold.control <- gcrma(raw.cold.control)
> Computing affinities.Done.
> Adjusting for optical 
> effect........................................................Done.
> Adjusting for non-specific 
> binding........................................................Done.
> Normalizing
> Calculating Expression
> Error in sigToEnv(signature, fdef) : Trying to get slot "signature" from 
> an object of a basic class ("NULL") with no slots
> 
> 
> The raw.cold.control object is:
> AffyBatch object
> size of arrays=712x712 features (221801 kb)
> cdf=ATH1-121501 (22810 affyids)
> number of samples=56
> number of genes=22810
> annotation=ath1121501
> notes=Merge from two AffyBatches with notes: 1) Merge from two 
> AffyBatches with notes: 1) Merge from two AffyBatches with notes: 1)  , 
> and 2)  , and 2)  , and 2)
> 
> R version 2.0.1
> gcrma versionl 1.1.3
> 
> I would really appreciate some feedback on the possible cause of the errors.
> 
> Dion Whitehead
> 
> -- 
> ***************************
> Dion Whitehead
> Division of Bioinformatics
> The Westfalian Wilhelms University of Muenster
> Botanical Institute, Schlossplatz 4
> D48149 Muenster
> Germany
> 
> Phone: +49-251-83-21633
> Fax:   +49-251-83-21631
> http://www.uni-muenster.de/Biologie.Botanik/ebb/
> 
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