[BioC] How can I extract Raw Intensities with Probe IDs on Affy Chips

Saurin Jani saurin_jani at yahoo.com
Tue Jan 11 16:52:30 CET 2005


Hi,

Is there any function which extracts raw intensities
and probids from Affy Chips?

I have 4 affy chips; 2 control and 2 experimental

> getIntensityMatrix(exprs(affybatch));
> it gives different output......??

or should I do RMA which converts log base 2 values
and  then take antilog but..its still confusing..!!

> do RMA(affybatch)
> MatrixRMA <- exprs(myRMA);
> MeanRawIntensities <-
as.matrix(rowMeans(2^MatrixRMA[,1:2]));

Can anyone guide me through this....?

Thank you,
Saurin



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