[BioC] RT-PCR normalization & stats reference

Ken Termiso jerk_alert at hotmail.com
Thu Mar 24 23:44:47 CET 2005

Hi all,

Anyone have a good reference for how to normalize and determine significance 
of fold change for RT-PCR data?

Basically, we have 6 control individuals and 6 experimentals. We normalize 
using 18s, but our RNA measurements going into the machine are pretty noisy, 
so we need to know what people who **successfully** confirm microarray data 
with RT-PCR are up to...


More information about the Bioconductor mailing list