[BioC] Dealing with technical replication with a common reference design
michael watson (IAH-C)
michael.watson at bbsrc.ac.uk
Mon Sep 12 12:43:58 CEST 2005
When I called duplicateCorrelation, I got three warning messages:
Too much damping - convergence tolerance not achievable in: glmgam.fit(
My corfit$consensus was then positive, even though it is a dye-swap
experiment. Presumably this means I don't have to treat the technical
replicates in any special way...?
From: Gordon Smyth [mailto:smyth at wehi.edu.au]
Sent: 11 September 2005 08:31
To: michael watson (IAH-C)
Cc: bioconductor at stat.math.ethz.ch
Subject: [BioC] Dealing with technical replication with a common
>Date: Fri, 9 Sep 2005 09:09:02 +0100
>From: "michael watson \(IAH-C\)" <michael.watson at bbsrc.ac.uk>
>Subject: [BioC] Dealing with technical replication with a common
> reference design
>To: <bioconductor at stat.math.ethz.ch>
>I have to say the limma userguide is now one of the most comprehensive
>guides available for microarray analysis. It is therefore regretfully
>that I ask for additional information...
>The guide rightly points out that we must not treat arrays that are
>technical replicates as true replicate arrays as they are not
>independent. Sections 10.1 and 10.2 then go on to explain how
>technical replicates can be handled using either duplicateCorrelation
>or by manipulating the contrasts matrix. However, both these examples
>deal with arrays where direct comparisons have been made.
>I have data from a common reference design, comparing two groups using
>a common reference, with biological replicates and technical replicates
>(dye swaps) and it's not immediately clear from the examples how I
>should deal with the technical replication.
The treatment of technical replicates is the same for common reference
designs as it is for direct designs. If all your technical reps are in
dye-swap pairs then the example in Section 10.1 with dye-swap technical
replicates will carry over to your situation with very few changes.
>Many thanks for any hints forthcoming!
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