[BioC] How use the function "normalizeWithinArrays" with loess in limma

sysra sysra at laposte.net
Mon Apr 24 17:09:05 CEST 2006


Hey,

I'm french student and it's my first mail on bioconductor,

I just want to use the loess normalization on my data, but i
don't understand which type of data the function
"normaliseWithinArrays" want. And, if i must apply the
function log, before or after normalisation.

So i'm a little lost.

(no log) (no background) (raw data)

my data profile : 
dat$R  dat$G   
49     35                      
118    34
29     101.5 
42     46.5
44     287.5
31     174 
   
my script :
MA <- normalizeWithinArrays(don, method="loess")

thinks,

Sylvain



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