[BioC] presence/absence call using oligo array?
xpeng at utk.edu
Fri Jan 13 09:07:13 CET 2006
Has someone ever used long oligo arrays to test if genes were expressed or
not? I have two microarray slides on which oligonucleotides (65mer) for genes
from a parasite genome were spotted. One gene has one oligo but spotted twice
on each array. One slide has a RNA sample extracted from infected tissues
(therefore mixture of both parasite RNAs and RNAs from host tissues)
co-hybridized with RNAs from uninfected host tissue (no parasite RNAs).
Another is a biological replicate but with dye-swap. People want to know what
parasite genes are expressed in the infected tissue. Is it possible to do so?
Related publications are highly appreciated.
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