[BioC] log2 ratio as limma input

yuhong ning yhning at yahoo.com
Fri Jun 16 18:07:52 CEST 2006


Hi,

I am trying to use limma to analyze Mass Spec data for
differentially expressed proteins from wild type cell
and mutant cells. The input data I got from scientists
is the intensity ratio of Heavy-isotope/light-isotope
peptide. I want to read it into MAList, but seems the
ratio data only correspondent to M, I have no way to
obtain the "A" data. What should I do?


Thanks a lot.

yuhong



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