[BioC] Unable to get expression-values from cel-files of yeast

Roman Brunnemann brunnemann at mpimp-golm.mpg.de
Fri Mar 3 16:14:35 CET 2006


Hi.

I'm working with bioconductor since 2 month and I never had any
problems. I used CEL-Files from ATGenExpress and everything was okay.
Now I tried to analyse different CEL-Files of yeast. I used different
sources (

http://chemogenomics.stanford.edu/supplements/01yfh/files/raw_data/tar_archives/
http://www.brownlab.info/publications/Brown2005/celfiles/index.htm
)

But I didn't succeeded to make an 

library(affy) and than data <- justRMA() in the cel-file directory.
On two different machines I got two different errors. The first computer
says:

##########################

> data <- justRMA()
Error in getCdfInfo(object) : Could not obtain CDF environment, problems
encountered:
Specified environment does not contain DNA_TAGS_3
Library - package dnatags3cdf not installed
Data for package affy did not contain dnatags3cdf
Bioconductor - dnatags3cdf not available


##########################

and the second one says:

##########################

library(affy)

Loading required package: Biobase

Loading required package: tools

Welcome to Bioconductor

Vignettes contain introductory material. To view,

simply type: openVignette()

For details on reading vignettes, see

the openVignette help page.

Loading required package: reposTools

> data <- justRMA()

Note: BIOCRel1.8/src/contrib does not seem to have a valid repository,
skipping

Warning:

Failed to read replisting at BIOCRel1.8/src/contrib in:
getReplisting(repURL, repFile, method = method)

Note: BIOCRel1.8/bin/windows/contrib/2.3 does not seem to have a valid
repository, skipping

Warning:

1: Failed to read replisting at BIOCRel1.8/bin/windows/contrib/2.3 in:
getReplisting(repURL, repFile, method = method)

2: Failed opening: HTTP Status war '404 Not Found'

Note:
http://www.bioconductor.org/packages/data/annotation/stable/bin/windows/contrib/2.3 does not seem to have a valid repository, skipping

Note: Using download type: Source

Fehler in getCdfInfo(object) : Could not obtain CDF environment,
problems encountered:

Specified environment specified did not contain DNA_TAGS_3

Library - package dnatags3cdf not installed

Data for package affy did not contain DNA_TAGS_3

Bioconductor - dnatags3cdf not available

Warning:

Failed to read replisting at
http://www.bioconductor.org/packages/data/annotation/stable/bin/windows/contrib/2.3 in: getReplisting(repURL, repFile, method = method)
##############################


Maybe anyone had the same problem allready and can help me out of this.

Thanks in advance.

Best regards. Roman Brunnemann



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