[BioC] Limma missing values

Gordon K Smyth smyth at wehi.EDU.AU
Tue Mar 7 13:00:19 CET 2006


> Date: Mon, 6 Mar 2006 14:45:13 +0100 (CET)
> From: kfbargad at ehu.es
> Subject: Re: [BioC] Limma missing values
> To: bioconductor at stat.math.ethz.ch
>
> Dear Gordon,
>
> thanks for your answer. There was a problem with the format, I had
> commas instead of dots for decimal separators. Sorry about that silly
> mistake, I should have checked all the possible options before posting
> my email. It now works fine
>
> Nevertheless, do you think that using limma for Q-PCR on 96 genes and
> 19 samples (two conditions) is a valid idea? Maybe it would be more
> preferable to use some other method instead?

In principle, limma should work fine on such data.

Best wishes
Gordon

> David



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