[BioC] Newbie 'rflowcyt' question; problem loading facscan256.fcs

Nolwenn LeMeur nlemeur at fhcrc.org
Mon May 29 22:15:12 CEST 2006


Hi Seth, Hi Ulrik,

I have verified the md5 hash of the data file on my system and I get the 
same signature as Seth:

> library("digest")
> datafile=system.file("fcs/facscan256.fcs",package="rfcdmin")
> digest(datafile,file=TRUE)
[1] "bfc603037460c0023569159c7360be0c"



Nolwenn

***********************************
Nolwenn Le Meur, PhD
Fred Hutchinson Cancer Research Center
Computational Biology
1100 Fairview Ave. N., M2-B876
P.O. Box 19024
Seattle, WA 98109-1024

On Mon, 29 May 2006, Seth Falcon wrote:

> Nolwenn LeMeur <nlemeur at fhcrc.org> writes:
> > Hi Ulrik,
> >
> > I was not able to reproduce your error. 
> 
> I wonder if everyone is using the exact same dataset?
> 
> > Ulrik Stervbo wrote:
> >>Here's what I've done after starting R:
> >>  
> >>
> >>>library(rflowcyt)
> >>>library(Biobase)
> >>>fcs.loc <- system.file("fcs", package = "rfcdmin")
> >>>file.location <- paste(fcs.loc, "facscan256.fcs", sep = "/")
> >>>FC.FCSRobj <- read.FCS(file.location, UseS3 = TRUE, MY.DEBUG =
> >>>TRUE,fcs.type = NULL)
> 
> Here's one way to verify:
> 
> 1. Install the digest package.  From R, do:
> 
>      source("http://bioconductor.org/biocLite.R")
>      biocLite("digest")
> 
> 2. Obtain the md5 hash of the data file.  By comparing this output,
>    we'll know whether everyone has the same file to begin with.
> 
>      library("rflowcyt")
>      library("digest")
>      
>      ## Get the path to the data file:
>      dataFile <- system.file("fcs/facscan256.fcs", package="rfcdmin")
>      > dataFile
>      [1] "/Users/seth/RLIB-2.4/rfcdmin/fcs/facscan256.fcs"
> 
>      ## Compute the md5 hash of the file, you should get the _same_
>      ## string as I do here.  If not, we have different files.
>      > digest(dataFile, file=TRUE)
>      [1] "bfc603037460c0023569159c7360be0c"
> 
> + seth
> 
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