[BioC] Segmentation Demo - problem in normalization

[Krys Kelly]

I am trying to run the Segmentation Demo. I have been cutting and pasting
the commands from the Segmentation Demo document. 

I get an error during the normalization.  When I run this command:

xn = normalizeByReference(davidTiling[, isRNA], davidTiling[, isDNA], pm =
PMind, background = isbg)

I get the following error message:

The likelihood optimization did not converge. A likely reason is that the
normalization parameters are not uniquely identifiable from the provided
data.
Possibly, the columns of the data matrix are exactly co-linear or affine
dependent - please verify the data to make sure there were no mix-ups.

Please can you help.

Thanks

Krys


Dr Krystyna A Kelly
University of Cambridge
Department of Pathology
Molteno Building, Tennis Court Road
Cambridge CB2 1QP
Tel:    01223 333331
Email: kak28 at cam.ac.uk