[BioC] MBEI on 60 U133 Plus 2.0 arrays

James MacDonald jmacdon at med.umich.edu
Tue Mar 6 19:49:39 CET 2007


Hi Kamila,

Currently you are limited by the amount of RAM you have. Technically you _could_ write a just.li.wong() that bypasses the explicit creation of an AffyBatch (as do justRMA() and justGCRMA()), and quite possibly you would be able to get away with just 2Gb RAM.

Best,

Jim


-- 

James W. MacDonald, M.S.
Biostatistician
Affymetrix and cDNA Microarray Core
University of Michigan Cancer Center
1500 E. Medical Center Drive
7410 CCGC
Ann Arbor MI 48109
734-647-5623

>>> On 3/6/2007 at 12:32 PM, <knaxerov at ix.urz.uni-heidelberg.de> wrote:
> Dear list,
> 
> I am wondering if I have any other normalization options besides  
> justRMA() and justGCRMA() if I want to normalize 60 U133 Plus 2.0  
> arrays on a machine with 2 GB of RAM. Since I run out of memory when  
> using ReadAffy on as few as 15 arrays, I suppose there really is no  
> way to use MBEI, for example, correct?
> 
> Thanks.
> Kamila
> 
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