[BioC] Problem in lumi

Longsi Ran longsiran1126 at hotmail.com
Mon May 7 20:27:55 CEST 2007


Hi,

When running lumi, I met some problems.  Here is the workflow:

>library(lumi)
Loading required package: Biobase
Loading required package: tools

Welcome to Bioconductor

  Vignettes contain introductory material. To view, type
  'openVignette()'. To cite Bioconductor, see
  'citation("Biobase")' and for packages 'citation(pkgname)'.

Loading required package: affy
Loading required package: affyio
Loading required package: mgcv
This is mgcv 1.3-23
Loading required package: annotate
>MyFileName <- 'in.txt'
>MyProject.lumi <- lumiR(MyFileName, lib='lumiHumanV1')
Loading required package: lumiHumanV1
Warning message:
Duplicated IDs found and were merged! in: addNuId2lumi(x.lumi, lib = lib)
>load(MyProject.lumi)
Error in load(MyProject.lumi) : bad 'file' argument
>data(MyProject.lumi)
Warning message:
data set 'MyProject.lumi' not found in: data(MyProject.lumi)

There are 47293 targetIDs with 17 columns in the BeadStudio output text 
file:
TargetID	MIN_Signal-1523532052_A	AVG_Signal-1523532052_A	MAX_Signal-1523532052_A	NARRAYS-1523532052_A	ARRAY_STDEV-1523532052_A	BEAD_STDEV-1523532052_A	Avg_NBEADS-1523532052_A	Detection-1523532052_A	MIN_Signal-1523532052_C	AVG_Signal-1523532052_C	MAX_Signal-1523532052_C	NARRAYS-1523532052_C	ARRAY_STDEV-1523532052_C	BEAD_STDEV-1523532052_C	Avg_NBEADS-1523532052_C	Detection-1523532052_C

No Duplicated IDs were found before loading the data.

Any help will be appreciated.

Longsi Ran M.D.

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