[BioC] One-Color Aglent Data into Limma GUI

[elliot harrison]

Hi All,
 
I'm trying to work with One-color agilent data without much luck.
http://article.gmane.org/gmane.science.biology.informatics.conductor/128
18/match=agilent 

I've tried the above method but as I posted before I get stuck on the
following error.
 
Error in readGenericHeader(fullname, columns = columns, sep = sep) : 
Specified column headings not found in file

As such I thought I'd try a different approach and have a go with the
LimmaGUI.
I've faked green channel columns in my files like this which I hope to
ignore once imported.

FEATURES	FeatureNum	Block	Row	Col	SubTypeMask
SubTypeName	ProbeUID	ControlType	ProbeName	GeneName
	SystematicName	PositionX	PositionY	gProcessedSignal
rProcessedSignal	gMeanSignal	rMeanSignal
	gMedianSignal	rMedianSignal	gBGUsed	rBGUsed
DATA	1	1	1	1	0		0	1
GE_BrightCorner	GE_BrightCorner	GE_BrightCorner	9790.72	223.949	358.8079
1.52E+04	1.10E+04	1.10E+04	10446	10446	78.8743
78.8743

When I try to import the files I gives an error saying it was unable to
read the image processing files

I've also added a Block column as the next error is in getLayout(gal)
GAL file must contain Block, Column, Row error.
Then Error in Matrix and other but I guess this is all due to the file
not being read properly in the first place.

I am using another analysis package as well so I am analysing my data
but there is so much more to R. 
Any help getting going would be greatly appreciated.


Many Thanks

Elliott


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