[BioC] RMA bgcorrection question

[Ben Bolstad]

No you don't look like an idiot and you are not doing anything wrong.
What you are seeing is quite common from the standard implementation.

A previous thread discussing this issue

https://stat.ethz.ch/pipermail/bioconductor/2006-June/013276.html

Best,

Ben



On Tue, 2008-02-05 at 19:06 -0800, Siobhan A. Braybrook wrote:
> Dear all,
> 
> At the risk of looking like an idiot:
> is rma background correction supposed to make log-intensity histograms bimodal?
> I noticed this in my own data, then checked it using the Dilution set too 
> (see below).
> This seems weird to me........am I doing something wrong?
> 
> data(Dilution)
> dil.rma<- bg.correct(Dilution, method="rma")
> hist(Dilution[,1:2])
> hist(dil.rma[,1:2])
> 
> 
> Thanks!
> Siobhan
> 
> S. A. Braybrook
> Graduate Student, Harada Lab
> Section of Plant Biology
> University of California,  Davis
> Davis, CA 95616
> Ph 530.752.6980
> 
> The time is always right, to do what is right.
> - Martin Luther King, Jr.  
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> 
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