[BioC] LIMMA: Duplicate spots

Cecilia McGregor cmcgre1 at lsu.edu
Wed Jul 23 22:04:49 CEST 2008

Hi All

I haven't used LIMMA for a while, but I remember that when I did 
there was a special way to handle duplicate spots. However the 
layout of the array had to be such that one replicate was printed 
first for all spots and then the other replicate, because somewhere 
you had to say something like there are 5000 spots and the next rep 
starts at spot number 5001. Is this still true? The reason I'm 
asking is because I'm developing Agilent arrays and they randomize everything so it would not be possible to say the duplicate spots start at a specific place.


Cecilia McGregor, Ph.D.
Department of Entomology
404 Life Sciences Building
Louisiana State University
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LA, 70803

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