[BioC] get probe level intensity from Affymetrix Exon Array platform

James W. MacDonald jmacdon at med.umich.edu
Tue Mar 18 17:47:37 CET 2008


I don't think you can. The exonmap package uses modified cdfs that have 
all the background and control probesets removed.

Best,

Jim



shirley zhang wrote:
> Hi Jim,
> 
> Thanks for your help. Can you also tell me how to get all of the
> background probe and positive/negative control probe data using the
> exonmap package?
> 
> Shirley
> 
> On Mon, Mar 17, 2008 at 10:14 AM, James W. MacDonald
> <jmacdon at med.umich.edu> wrote:
>> Hi Shirley,
>>
>> You can get the probe-level data using the exonmap package.
>>
>> Best,
>>
>> Jim
>>
>>
>>
>>
>> shirley zhang wrote:
>>> Dear Bioconductor,
>>>
>>>  I usually obtain exon-level/gene-level signal using Plier/RMA
>>> implemented in Affymetrix's Expression Console, however there is no
>>> way to get probe level intensity from it. Does anybody know how to get
>>> probe level intensity from Affymetrix Exon Array platform?
>>>
>>> Thanks,
>>> Shirley
>>>
>>> _______________________________________________
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>> --
>> James W. MacDonald, M.S.
>> Biostatistician
>> Affymetrix and cDNA Microarray Core
>> University of Michigan Cancer Center
>> 1500 E. Medical Center Drive
>> 7410 CCGC
>> Ann Arbor MI 48109
>> 734-647-5623
>>

-- 
James W. MacDonald, M.S.
Biostatistician
Affymetrix and cDNA Microarray Core
University of Michigan Cancer Center
1500 E. Medical Center Drive
7410 CCGC
Ann Arbor MI 48109
734-647-5623



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