[BioC] Input to vsn or limma of genepix file missing 635 columns

[Liolios, Konstantinos [BSD] - MED]

Dear bioconductors,

I have been given Exiqon miRCURY(tm) LNA Array (miRNA) expression gpr
files in excel format.  They are single channel so they are actually
missing all 635 related (mean/median/back/for etc.) columns.  I believe
vsn is the way to normalize these data but I can not find anywhere how
to import such a file.  Most tutorials use data that already come with
the package or assume you have .cel files and an affymetrix annotation
file.  I have used read.table which creates a data.frame but most
normalization packages require you to have your data as an expression
set.  I was wondering if somebody knows how to import single channel gpr
files or how to manually create an expression set from a data.frame w/o
an annotation file.

Best regards
Dinos


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