[BioC] Nimblegen Microarray-gene expression analysis using Biocnductor
richmond.todd at gmail.com
Wed Oct 8 02:57:57 CEST 2008
Dhiraj Naik wrote:
> I am postdoc working with genome wide gene expression using Poplar
> oligonucleotide Nimblgen array. I am beginner in R and bioconductor. I have
> Nimblgen raw data in .pair file format. I dont know how to use that using
> Oligo vignette which is available in bioconductor. How to have input file in
> R format from .pair files?
The oligo package loads .xys files, not .pair files. If you did the
hybridizations in your own lab, use NimbleScan to generate .xys files
from the tiff images. If you got your data from the service lab, contact
technical support and ask them to generate the .xys files for your order.
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