[BioC] Non linear intensities
vilanew at gmail.com
Thu Jul 23 17:25:25 CEST 2009
I have a naive question. In my experiment i have computed different
standard curves for different genes.
I have noticed that my data is not following a linear pattern (sorry i'm
not statistician so don't know if this is the word).
GENES [1µg] [2µg] [5µg] [10µg]
gene A 500 800 1800 3500
gene B 450 650 1700 3400
gene C 200 300 600 1300
As you can see the intensities are not as I would expect (there is no
linear intensity based on the concentration); Since my data shows that
there is a bias i would like to correct the data and adjust according.
For instance, i think there might be a coeffecient, which based on the
intensitiy would help to correct the intenstities. At the end if for a
gene X i find an intensity of 500 i could assume that it should higher
than that to have a 2 fold change and that it should probably be
somewhere around 1000.
Thanks for your help ??
thanks for your help.
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