[BioC] MAS5 to selected average

James W. MacDonald jmacdon at med.umich.edu
Mon Jun 1 15:39:10 CEST 2009


Hi Balazs,

To which MAS5 do you refer? The implementation in Affy's software, or 
the mas5() function in the affy package?

If mas5(), then the scale value is set at 500, so there shouldn't be a 
difference between any chip type. See ?affy.scalevalue.exprSet.

Best,

Jim



Dr Balazs Gyorffy wrote:
> Hi!
>  
> MAS5 normalizes to different averages when using HGU133A (to 800) and HGU133Aplus2 (to 1500) chips. Can I set the target average during normalization?
>  
> Thanks
> Balazs
> 
> 
>       
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-- 
James W. MacDonald, M.S.
Biostatistician
Douglas Lab
University of Michigan
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