[BioC] filetr to Agilent 4 x 44 array

Wei Shi shi at wehi.EDU.AU
Thu Feb 18 22:35:59 CET 2010


Hi,

    Maybe another way is to use the detection call if it is available in 
the array.

Cheers,
Wei

Francois Pepin wrote:
> Hi Marco,
>
> I have done something in the past at that particular cut-off.
>
> I would suggest to take a look at your distribution of intensities to 
> make sure that 6 is a reasonable threshold. I tend to have bimodal 
> distributions with a sharp peak just below 6 and another later on, but 
> your data might be different.
>
> Another strategy is to use the Limma F statistics, if you want to also 
> include genes which might be more highly expressed but also don't have 
> any variations.
>
> Hope this helps,
>
> Francois
>
> On Feb 18, 2010, at 1:45 AM, Marco Fabbri wrote:
>
>> HI all,
>>    I remeber that when I worked with affy chip , removing those genes
>> that appear not to
>> be expressed in either sample, value smaller than 6.
>> I was wondering if I can apply a similar filter to Agilent 4 x 44 gene
>> expression arrays.
>>
>> THank you
>>
>> MArco
>>
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