[BioC] RMA without probe summarization

James W. MacDonald jmacdon at med.umich.edu
Wed May 26 15:14:42 CEST 2010


Hi Sneha,

sneha patil wrote:
> Hello ,
> 
> I have checked the mailer list n got to know that some one have asked about
> the RMA step without summarization so that you could get the probe level
> data.....have anyone solved it?? If yes please can u let me know which code
> you used for it...Thak you..

This has been asked and answered many times, so maybe you need better 
search terms. Anyway, it's very simple.

abatch <- ReadAffy()
bgcorrected <- bg.correct(abatch, "rma")
normalized <- normalize(bgcorrected, "quantiles")

Best,

Jim




> 
> Regards,
> Sneha.
> 
> 	[[alternative HTML version deleted]]
> 
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-- 
James W. MacDonald, M.S.
Biostatistician
Douglas Lab
University of Michigan
Department of Human Genetics
5912 Buhl
1241 E. Catherine St.
Ann Arbor MI 48109-5618
734-615-7826
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