[BioC] Package plgem for analysis of spectral counts, was 'limma for spectral counts'

Ina Hoeschele inah at vbi.vt.edu
Mon Oct 25 16:34:22 CEST 2010

Hi all,
  I am using SAFE for testing gene functional categories for the first time. I have some issues:
(1) my dataset has only small numbers of replicates, 2 to 4. I have therefore used limma for differential expression analysis. SAFE does not include the limma t-statistic, but allows for user-defined (local) statistics. Is it possible to use limma in SAFE, has anyone done this? If not, how bad is it to use the standard t-stat? I've compared limma with standard t (with low variance pre-filtering) and limma clearly had more significant (FDR) results. 
(2) Alternatively, is it possible to just input p-values into SAFE (of course cannot do permutations then)?
Thanks, Ina

More information about the Bioconductor mailing list