[BioC] HTqPCR plotCtRep only accepts 2 replicates
kee.wong at wafergen.com
Fri Sep 17 19:14:11 CEST 2010
Thank you very much for your quick reply.
What you have listed is exactly what I was looking for. Your help is greatly appreciated.
From: Heidi Dvinge [mailto:heidi at ebi.ac.uk]
Sent: Friday, September 17, 2010 10:10 AM
To: Kee Wong
Cc: bioconductor at stat.math.ethz.ch
Subject: Re: [BioC] HTqPCR plotCtRep only accepts 2 replicates
> Dear list members,
> Does anyone know if there are any functions like plotCtRep that can take
> more than two technical replicates and look at the SD of the reps?
There currently isn't any function in HTqPCR for doing that - mainly since
I've only worked with qPCR plates with max 2 replicates myself.
However, I've been toying with the idea of adding something like a plot of
mean (or median) versus standard deviation. Or perhaps just something like
a boxplot of standard deviations within each plate. Would that be useful,
or was it something else you had in mind?
> Kee Wong
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