[BioC] HTqPCR plotCtRep only accepts 2 replicates

Kee Wong kee.wong at wafergen.com
Fri Sep 17 19:40:06 CEST 2010


Thank you Heidi. You're the best!

Kee

-----Original Message-----
From: Heidi Dvinge [mailto:heidi at ebi.ac.uk] 
Sent: Friday, September 17, 2010 10:32 AM
To: Kee Wong
Cc: bioconductor at stat.math.ethz.ch
Subject: RE: [BioC] HTqPCR plotCtRep only accepts 2 replicates

Ok. It'll probably take me a while to get some proper functions into the
package, but I can send you some preliminary versions off-list within the
next couple of days, so you can test them and see if they're suitable for
you data/purpose.

Cheers
\Heidi

> Hello Heidi,
>
> Thank you very much for your quick reply.
>
> What you have listed is exactly what I was looking for. Your help is
> greatly appreciated.
>
> Best,
>
> Kee
>
> -----Original Message-----
> From: Heidi Dvinge [mailto:heidi at ebi.ac.uk]
> Sent: Friday, September 17, 2010 10:10 AM
> To: Kee Wong
> Cc: bioconductor at stat.math.ethz.ch
> Subject: Re: [BioC] HTqPCR plotCtRep only accepts 2 replicates
>
> Hello Kee,
>
>> Dear list members,
>>
>> Does anyone know if there are any functions like plotCtRep that can take
>> more than two technical replicates and look at the SD of the reps?
>>
> There currently isn't any function in HTqPCR for doing that - mainly since
> I've only worked with qPCR plates with max 2 replicates myself.
>
> However, I've been toying with the idea of adding something like a plot of
> mean (or median) versus standard deviation. Or perhaps just something like
> a boxplot of standard deviations within each plate. Would that be useful,
> or was it something else you had in mind?
>
> Cheers
> \Heidi
>
>> Best,
>>
>> Kee Wong
>>
>>
>> 	[[alternative HTML version deleted]]
>>
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>
>
>



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