[BioC] Manual creation of Affybatch object to study highthrouput qPCR experiments.

[James Perkins]

One could use read.qpcr in the new ReadqPCR package, currently in
R-devel, to read the data into an eset extending qPCRBatch. You could
then normalise with an endogenous control, although I'm not sure how
appropriate this is when using miRNA, if you haven't a priori selected
a reference.

Potentially useful: www.ncbi.nlm.nih.gov/pubmed/18375788

Cheers,

Jim

On 20 October 2011 07:49, Ali Mo [guest] <guest at bioconductor.org> wrote:
>
> Hi every one.
>
> I am using 384 well qPCR array for miRNA study. I wanted to normalize my results using affy. How can I create an affybatch wich contains my Cq values? Thanks a lot.
>
> PS. I am new to R/bioconductor.
>
>  -- output of sessionInfo():
>
> No R command!
>
> --
> Sent via the guest posting facility at bioconductor.org.
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