[BioC] annotation file and expression set genechip miRNA 2 array

Attia [guest] guest at bioconductor.org
Fri Oct 21 14:10:12 CEST 2011


Hi Sean 
Thanks for your response but  I did not get how does it work  after I use read.table to read the annotation file in to a data frame 
For miRNA 2.0 Array  annotation file  from affymatrix siteFOR my miRna data
spleen.affy = ReadAffy()
sprma<-rma(spleen.affy)
pData(spleen.affy)[,1]<-c("N","N","N","P","P","P","P","P","P","N","N","N")

exprs(sprma)
here I want to apply annotation file read in data frame 
to  my data how to go about it
thanks 

 -- output of sessionInfo(): 

> sessionInfo()
R version 2.13.1 (2011-07-08)
Platform: x86_64-pc-mingw32/x64 (64-bit)

locale:
[1] LC_COLLATE=English_Ireland.1252  LC_CTYPE=English_Ireland.1252   
[3] LC_MONETARY=English_Ireland.1252 LC_NUMERIC=C                    
[5] LC_TIME=English_Ireland.1252    

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] mirna20cdf_2.8.0 affy_1.30.0      Biobase_2.12.2  

loaded via a namespace (and not attached):
[1] affyio_1.20.0         preprocessCore_1.14.0 tools_2.13.1         
> 


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