[BioC] DESeq normalization

Simon Anders anders at embl.de
Wed Aug 8 12:08:12 CEST 2012


Hi Sudeep

On 2012-08-08 08:28, sudeep s wrote:
> As you said in the post, I was plotting to see if the size factor was
> hitting the median, in some samples (3), the size factor was way off
> the median and hence I tried shorth, but this didn't help either, and
> I tried filtering down the genes (filtering genes on row sums for
> 10,15,20, 25...) but again in the plot size factor was way off, so I
> was wondering what I should follow.

I guess you'd need to post your plots to the list. It's hard to advise 
without seeing them.

   Simon



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