[BioC] HT qPCR; Creating qPCRset from expression matrix

Heidi Dvinge heidi at ebi.ac.uk
Wed Aug 8 19:31:35 CEST 2012


> Hi all!
>
> How is it possible to create a qPCRset object from an expression
> matrix (in new veersions of HTqCR?)
>
Basically the same way as before ;)

There's an example is the vignette, but in the (admittedly not very
informative titled) "Additional devices" section. E.g.

mat <- matrix(rnorm(9*96), ncol = 6, nrow = 96,  byrow = FALSE)
raw <- new("qPCRset", exprs = mat, featureCategory =
as.data.frame(array("OK",  dim=dim(mat))))
sampleNames(raw) <- paste("S", 1:6, sep = "")
featureNames(raw) <- paste("A", 1:96, sep = "")

This is the simplest approach. Alternatively, if you e.g. want more
information about your samples, you can add phenoData to the object either
during or after creating it. See ?'qPCRset-class' for more details about
the qPCRset objects.

HTH
\Heidi

> Thank you indeed very much.
>
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