[BioC] limma

Sean Davis sdavis2 at mail.nih.gov
Mon Aug 20 23:08:05 CEST 2012


On Mon, Aug 20, 2012 at 5:03 PM, Novak Jaroslav
<jaroslav.novak at gmail.com> wrote:
> I want to use limma (3x2 factorial experiment), I have a very large
> data set, which cannot be handled as CEL files by my computer.
> However, I have all expression values. How can I create eset file
> using my summary expression values? Thanks for help! /Jaroslav

Hi, Novak.

There really isn't a need to create an ExpressionSet for the purposes
of running limma.  Limma is quite happy with using a matrix as input.
If you do want to create an ExpressionSet, be sure to read the
documentation associated with the Biobase package.

Sean



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