[BioC] about library size and length of gene information in DEseq

[Wolfgang Huber]

Dear Peter

wang peter scripsit 02/10/2012 05:05 PM:

> i am suing DEseq,

Please don't. What has it done to you.

> but i donot know
> how to set length of gene information,
> does it use length information in finding differential expressed genes?

It does not.

> and either i donot know how to set library size of each sample
> but in edgeR, I can do it by such function
> d<- DGEList(counts = d, lib.size =
> c(9893630,11055814,11207084,9663487,11455088,8140053), group = group)
>

Have a look at the package vignette. After that, you can type:

  ? `sizeFactors<-`

Best wishes

	Wolfgang

Wolfgang Huber
EMBL
http://www.embl.de/research/units/genome_biology/huber