[BioC] regenerate CEL file from analyzed data

Kasper Daniel Hansen kasperdanielhansen at gmail.com
Wed Jul 11 19:03:46 CEST 2012


You cannot do this.

Kasper

On Wed, Jul 11, 2012 at 12:16 PM, J. Greenbaum <jgbaum at liai.org> wrote:
> Thanks Benilton,
>
> All I have is a list of probeset names and the normalized intensity values...no probe level data. So what I would need to do is to generate the probe level data that would produce these normalized intensity values and then use createCel. Has anyone attempted this before?
>
> -J
>
> -- Jason Greenbaum, Ph.D.
> Manager, Bioinformatics Core | jgbaum at liai.org
> La Jolla Institute for Allergy and Immunology
>
> ----- Original Message -----
>
>> From: "Benilton Carvalho" <beniltoncarvalho at gmail.com>
>> To: "J. Greenbaum" <jgbaum at liai.org>
>> Cc: bioconductor at r-project.org
>> Sent: Wednesday, July 11, 2012 3:14:37 AM
>> Subject: Re: [BioC] regenerate CEL file from analyzed data
>
>> Really depends on the definition of "processed data". If by that you
>> mean that the user only has the results after something like RMA,
>> there's nothing you can do to generate a CEL file from that.
>
>> If the user has the intensity values prior to preprocessing (ie.,
>> probe-level data), then one suggestion is to use the affxparser
>> package (createCel/updateCel).
>
>> b
>
>> On 11 July 2012 03:55, J. Greenbaum <jgbaum at liai.org> wrote:
>> > Hi all,
>> >
>> >
>> > I have an odd request....
>> >
>> >
>> > I was wondering if there is a way to create a CEL file from
>> > processed data. We have a user here who needs to deposit their
>> > data in GEO for publication, but they have lost their original CEL
>> > file as the experiments were done 8 years ago. So, I was wondering
>> > if any of the Bioconductor modules (or any tool at all) might have
>> > the functionality to take a list of probe sets and intensity
>> > values and create a CEL file. I realize this is a long shot, but I
>> > thought I would throw it out there.
>> >
>> >
>> > Thanks in advance!
>> >
>> >
>> > -Jason
>> >
>> >
>> >
>> >
>> >
>> >
>> > -- Jason Greenbaum, Ph.D.
>> > Manager, Bioinformatics Core | jgbaum at liai.org
>> > La Jolla Institute for Allergy and Immunology
>> >
>> >
>> >
>> >
>> >
>> > [[alternative HTML version deleted]]
>> >
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>
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