[BioC] how to count for edgeR input

wang peter wng.peter at gmail.com
Mon Jul 23 15:59:54 CEST 2012


dear all:
       i have non-strand specific RNA-seq samples for
edgeR analysis.
      first, i used bowtie to map my reads to the
assembled contigs to count the sample. then for each
contig, i got two number. one is forward count, the
other is reverse count.
      if i sum these two number together to get one
count number for edgeR input. is it right?
      should i sum them or average them

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