[BioC] How to print out normalized Cy5 and Cy3 signals

Yong Li mail.yong.li at googlemail.com
Tue Jun 19 18:16:40 CEST 2012


Hi,

I think one additional step before doing what Belinda suggested is to
run RG.MA() because after normalization in limma you get MAlist and
Cy5 and Cy3 signals are not in MAlist. You need to convert it back to
RGlist.

Kind regards,
Yong

On Mon, Jun 18, 2012 at 2:54 AM, Belinda Phipson <phipson at wehi.edu.au> wrote:
> Hi Mei
>
> Check the names of your data object:
>> names(data)
> to figure out where the normalized data is and then use the
>> write.csv(data$...,file="norm.csv")
> which can write matrices or data frames to a file which can be opened in
> excel.
>
> Cheers,
> Belinda
>
> -----Original Message-----
> From: bioconductor-bounces at r-project.org
> [mailto:bioconductor-bounces at r-project.org] On Behalf Of JiangMei
> Sent: Saturday, 16 June 2012 5:05 AM
> To: bioconductor at r-project.org
> Subject: [BioC] How to print out normalized Cy5 and Cy3 signals
>
>
> Hi All. Sorry to bother you.
>
> I used limma package to normalize my two-color microarray data. I want to
> export the normalized Cy5 and Cy3 signals. Does anyone know how to do that?
> Thanks very much in advance.
>
>
> Best, Mei
>
>
>
>        [[alternative HTML version deleted]]
>
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