[BioC] eset -Affy Mouse Gene 1.0 ST. Array-

Djork-Arné Clevert okko at clevert.de
Thu May 10 13:40:48 CEST 2012


Hi Alex,

try to use the alternative CDFs from Brainarray. They provide a better probe mapping which is based on the latest genome and transcriptome information. 
Install the aCDFs of interest, change rawData at cdfName to the corresponding aCDF, afterwards run the summarization and that's all.

Cheers,
Okko


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Am 10.05.2012 um 12:43 schrieb alessandro brozzi:

> hi all,
> I'm working on Affy Mouse Gene 1.0 ST. Array.
> 
> If I do:
> 
> rawData = ReadAffy()
> eset = rma(rawData)
> 
> I end up with 34760 Features which correspond to transcript_cluster_id.
> Each transcript_cluster_id is formed by several probe_ids
> 
> How can I make an eset with the probe_id levels not summarized in
> transcript_cluster
> s?
> 
> thanks in advance,
> Alex
> *
> -----------------------------
> Alessandro Brozzi, PhD
> Department of Mathematics
> University of Perugia,
> Via Vanvitelli 1, 06123 Perugia, Italy
> tel: + 39 075 585 5039
> fax: **+ 39 075 585 5024
> *
> 
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