[BioC] Normalizing affymetrix chips with ERCC

Thornton, Matthew Matthew.Thornton at med.usc.edu
Tue Nov 20 01:26:39 CET 2012


Hello,

I was wondering if anyone had been using the ERCC controls from Ambion with Affymetrix human gene ST arrays? I guess there are probes sets on the chips for them, but there isn't much in the literature about anyone actually using them. I am thinking that it would be better to process the data using dchip, but I would rather use R and bioconductor.  Any comments are welcome!

TIA

Matt






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