[BioC] how to get normalized matrix

eyal avisar enfer0666 at yahoo.com
Thu Oct 4 16:21:10 CEST 2012


hello,
 
my name is Eyal and i am new to R, cut and paste level.
i have created a DGEList object - d following case study 3.3.3.
i ran the calcNormFactors(d).
the output i need is a normalized table.
 > library(edgeR)
> library(limma)
> raw.data <- read.delim("myFile.txt")
> names(raw.data)
 d <- raw.data[, 2:64]
 rownames(d) <- raw.data[, 1]
 
i did not group, irrelevant for me
 d <- DGEList(counts = d)
 dim(d)
 
result was [1] number... 3
 > cpm.d <- cpm(d)
> d <- d[ rowSums(cpm.d > 1) >=3, ]
> d <- calcNormFactors(d)
 
thx,
 
Eyal


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