[BioC] LIMMA: question about design and contrasts
James W. MacDonald
jmacdon at uw.edu
Fri Oct 19 15:51:35 CEST 2012
On 10/19/2012 1:10 AM, Patrick De Boever wrote:
> Dear all,
> We have used single color arrays for the following experiment:
> 25 individuals were split in 2 groups: location 1 and location 2. All individuals underwent an intervention.
> The individuals were analyzed twice: once before intervention and once after intervention.
> Which design should we use within LIMMA for assessing the effect of the location, the effect of the intervention, and possible interaction effect?
I think you will need to give more information. What do you mean exactly
by '25 individuals were split in 2 groups: location 1 and location 2.' ?
That can be interpreted several ways. Are the locations sampling
locations (e.g., one group was sampled from location 1 on their body,
and the other was sampled from location 2) or are these physical
locations (one group was recruited and samples were taken by lab 1 and
the other group went to lab 2, and the two labs are in different parts
of the country/university/world/whatever).
> Thank you for your kind reaction,
> Patrick De Boever, PhD, MSc
> Environment and Health Research Centre for Environmental Sciences
> VITO NV Hasselt University - Campus Diepenbeek
> Industriezone Vlasmeer 7, 2400 Mol, Belgium Agoralaan building D, 3590, Diepenbeek, Belgium
> Tel. + 32 14 33 51 45 patrick.deboever at uhasselt.be
> Fax. + 32 14 58 05 23
> patrick.deboever at vito.be
> Visit our website: www.vito.be/english
> Sent from my iPad
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James W. MacDonald, M.S.
University of Washington
Environmental and Occupational Health Sciences
4225 Roosevelt Way NE, # 100
Seattle WA 98105-6099
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